ISO TS 20224-5
Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by realtime PCR — Part 5: Goat DNA detection method
| Organization: | ISO |
| Publication Date: | 1 July 2020 |
| Status: | active |
| Page Count: | 22 |
| ICS Code (General methods of tests and analysis for food products): | 67.050 |
scope:
This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of goat-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of goat material derived from goat (Capra hircus).
The target sequence is a partial fragment of the goat chromosome 9 DNA sequence (i.e. GenBank accession number NC_030816.1)[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD95 %).
[1] Lowe T. M., Eddy S.R. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res. 1997, 25(5), pp. 955-964
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