scope:

This European Standard defines general rules for the enumeration of probiotic lactobacilli in feed samples (additives, premixtures and feeding stuffs) that contain lactobacilli as a single bacterial component or in a mixture with other microorganisms. This standard is not applicable to mineral feeds, which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40% crude ash (Council Directive 79/373/EEC [3]).

There are different categories of feed samples:

a) Additives containing about 10¹⁰ colony forming units (CFU)/g;

b) Premixtures containing about 10⁸ CFU/g;

c) Feeds, meal or pellets, which contain about 10⁶ CFU/g and include complete feeding stuffs and milk replacers.

The detection limit is as defined in EN ISO 7218.