DS/EN ISO 15216-1/A1
Microbiology of the food chain – Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR – Part 1: Method for quantification – Amendment 1 (ISO 15216-1:2017/Amd 1:2021)
active, Most Current
| Organization: | DS |
| Publication Date: | 27 April 2021 |
| Status: | active |
| Page Count: | 14 |
| ICS Code (Food microbiology): | 07.100.30 |
scope:
ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR. This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.
Document History
DS/EN ISO 15216-1/A1
April 27, 2021
Microbiology of the food chain – Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR – Part 1: Method for quantification – Amendment 1 (ISO 15216-1:2017/Amd 1:2021)
ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb...
April 3, 2017
Microbiology of the food chain – Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR – Part 1: Method for quantification (ISO 15216-1:2017)
ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb...
Microbiology of the food chain – Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR – Part 1: Method for quantification (ISO/FDIS 15216-1:2016)
This standard describes the detection of food-borne viruses in food based on PCR technique
Microbiology of the food chain – Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR – Part 1: Method for quantification – Amendment 1 (ISO 15216-1:2017/DAM 1:2020)
ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb...