CEN - PREN 17992
Food authenticity - Determination of the sum of 16-O-methylcafestol, 16-O-Methylkahweol and their derivatives in roasted coffee by ¹H-qNMR
| Organization: | CEN |
| Publication Date: | 1 June 2023 |
| Status: | pending |
| Page Count: | 25 |
| ICS Code (Coffee and coffee substitutes): | 67.140.20 |
scope:
This document specifies a method for the determination of soluble 16-O-Methylcafestol and 16-O-Methyl kahweol content (the sum of free forms and derivatives, e.g. fatty acid esters, henceforth abbreviated as 16-OMD = "diterpenes") in roasted coffee (beans or ground), using quantitative proton nuclear magnetic resonance spectroscopy (1H-qNMR).
If complying with the experimental parameters described below, this test procedure has been proven for the following concentration range:
16-OMD: 20 mg/kg to 2 000 mg/kg.
The concentration range can be expanded by suitable changes of the experimental parameters, e.g. a different weighed portion of ground coffee or the accumulation of more NMR-transients.
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