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ASTM International - ASTM E1532-00(2006)

Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of Bisbenzamide DNA-Binding Fluorochrome (Withdrawn 2014)

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Organization: ASTM International
Publication Date: 1 November 2006
Status: inactive
Page Count: 2
ICS Code (Medical microbiology): 07.100.10
significance And Use:

Mycoplasma hyorhinis, cultivar α strains (1)3 do not grow on any of the standard media used for mycoplasma cultivation. These strains, which are found as contaminants in cell... View More

scope:

1.1 This practice covers the use of cell cultures and DNA-binding flurorochrome techniques to detect mycoplasma contamination of cell cultures.

1.2 This practice does not cover axenic cultivation or identification of mycoplasmas.

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Document History

ASTM E1532-00(2006)
November 1, 2006
Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of Bisbenzamide DNA-Binding Fluorochrome (Withdrawn 2014)
Mycoplasma hyorhinis, cultivar α strains (1)3 do not grow on any of the standard media used for mycoplasma cultivation. These strains, which are found as contaminants in cell cultures, are detected...
May 10, 2000
Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of the Bisbenzamide DNA-Binding Fluorochrome
1.1 This practice covers procedures used for the detection of mycoplasma contamination by DNA staining. 1.2 This practice does not cover cultivation methods for the detection of mycoplasma or other...
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