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ASTM International - ASTM E1532-00

Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of the Bisbenzamide DNA-Binding Fluorochrome

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Organization: ASTM International
Publication Date: 10 May 2000
Status: inactive
Page Count: 2
ICS Code (Medical microbiology): 07.100.10
scope:

1.1 This practice covers procedures used for the detection of mycoplasma contamination by DNA staining.

1.2 This practice does not cover cultivation methods for the detection of mycoplasma or other methods such as biochemical detection, hybridization methodology, histochemical stains, or immunochemical methods.

1.3 This practice does not cover methods for the identification of mycoplasma organisms.

1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

1.5 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Document History

November 1, 2006
Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of Bisbenzamide DNA-Binding Fluorochrome (Withdrawn 2014)
Mycoplasma hyorhinis, cultivar α strains (1)3 do not grow on any of the standard media used for mycoplasma cultivation. These strains, which are found as contaminants in cell cultures, are detected...
ASTM E1532-00
May 10, 2000
Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of the Bisbenzamide DNA-Binding Fluorochrome
1.1 This practice covers procedures used for the detection of mycoplasma contamination by DNA staining. 1.2 This practice does not cover cultivation methods for the detection of mycoplasma or other...
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