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ASTM International - ASTM E1482-12

Standard Practice for Use of Gel Filtration Columns for Cytotoxicity Reduction and Neutralization

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Organization: ASTM International
Publication Date: 1 October 2012
Status: inactive
Page Count: 3
ICS Code (Disinfectants and antiseptics): 11.080.20
ICS Code (Chemicals for industrial and domestic disinfection purposes): 71.100.35
significance And Use:

4.1 This practice is to be used for the removal of virucidal agents from test product-virus mixtures, or from test product-neutralizer-virus mixtures, at or after the contact period and... View More

scope:

Note 1-The title was formerly Standard Test Method for Neutralization of Virucidal Agents in Virucidal Efficacy Evaluations.

1.1 This practice is intended to be used to reduce the cytotoxic level of the virus-test product mixture prior to assaying for viral infectivity. It is used in conjunction with evaluations of the virucidal efficacy of disinfectant solutions, wipes, trigger sprays, or pressurized disinfectant spray products intended for use on inanimate, nonporous environmental surfaces. This practice may also be used in the evaluation of hygienic handwashes/handrubs, or for other special applications. The practice may be employed with all viruses and host systems.

1.2 This practice should be performed only by persons trained in virology techniques.

1.3 This practice utilizes gel filtration technology. The effectiveness of the practice is dependent on the ratio of gel bed volume to sample size and uniformity in the preparation of columns as well as the conditions of entrifugation. The effectiveness of this practice is maximized by investigator practice and experience with gel filtration techniques.

1.4 This practice will aid in the reduction, but not necessarily elimination, of test product toxicity while preserving the titer of the input virus.

1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Document History

November 1, 2017
Standard Practice for Use of Gel Filtration Columns for Cytotoxicity Reduction and Neutralization
Note 1: The title was formerly Standard Test Method for Neutralization of Virucidal Agents in Virucidal Efficacy Evaluations. 1.1 This practice is intended to be used to reduce the cytotoxic level...
ASTM E1482-12
October 1, 2012
Standard Practice for Use of Gel Filtration Columns for Cytotoxicity Reduction and Neutralization
Note 1—The title was formerly Standard Test Method for Neutralization of Virucidal Agents in Virucidal Efficacy Evaluations. 1.1 This practice is intended to be used to reduce the cytotoxic level of...
October 1, 2004
Standard Test Method for Neutralization of Virucidal Agents in Virucidal Efficacy Evaluations
1.1 This test method covers the use, in conjunction with evaluations of the virucidal efficacy, of disinfectant solutions or pressurized disinfectant spray products intended for use on inanimate...
April 1, 2004
Standard Test Method for Neutralization of Virucidal Agents in Virucidal Efficacy Evaluations
1.1 This test method covers the use, in conjunction with evaluations of the virucidal efficacy, of disinfectant solutions or pressurized disinfectant spray products intended for use on inanimate...
October 10, 1998
Standard Test Method for Neutralization of Virucidal Agents in Virucidal Efficacy Evaluations
1.1 This laboratory test method is intended for use in conjunction with evaluations of the virucidal efficacy of disinfectant solutions or pressurized disinfectant spray products intended for use on...
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