VDI 4330 BLATT 7
Monitoring the effects of genetically modified organisms (GMO) - Qualitative methods for the detection of genetically engineered nucleic acids in the environment
| Organization: | VDI |
| Publication Date: | 1 December 2006 |
| Status: | active |
| Page Count: | 20 |
| ICS Code (Biology. Botany. Zoology): | 07.080 |
scope:
This guideline describes procedures for the detection of genetically modified organisms (GMOs) using the polymerase chain reaction (PCR). The described individual procedures are suitable for detecting gene constructs that exist frequently in GMOs ("screening"). Furthermore, references to other constructspecific or event-specific procedures can be found in the annexes.
Suitable methods for collection, transport, and storage of samples as well as for the extraction of nucleic acids will be given in guidelines VDI 4330 Part 5 and Part 6.
The procedure described in this guideline is suited, amongst other samples, for the analysis of DNA extracted from plant materials, soil, and compost. This method can also be applied to analyse DNA from other matrices, such as feed materials and foodstuffs.
Detailed information regarding specific procedures is given in the appendices.
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