Microbiology of the food chain - Horizontal method for the enumeration of beta-glucuronidasepositive Escherichia coli - Part 1: Colony-count technique at 44 °C using membranes and 5-bromo-4-chloro-3- indolyl beta-D-glucuronide
|Publication Date:||1 April 2018|
|ICS Code (Food microbiology):||07.100.30|
This document specifies a horizontal method for the enumeration of β-glucuronidase-posi
- products intended for human consumption,
- products intended for feeding animals,
- environmental samples in the area of food production and food handling, and
- samples from the primary production stage such as animal faeces, dust, and swabs.
WARNING - Some strains of Escherichia coli may grow poorly or not at all in media incubated at 44 °C. This includes strains of E. coli O157:H7 and O157:H-. Additionally, some strains of Escherichia coli, notably those belonging to serotype O157:H7, are mostly β-glucuronidase negative. Consequently, some strains of E. coli, including pathogenic ones, will not be detected by this method. β-glucuronidase activity may also be exhibited at 44 °C by certain other members of the Enterobacteriaceae, notably Shigella and Salmonella .