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ISO 16649-1

Microbiology of the food chain - Horizontal method for the enumeration of beta-glucuronidasepositive Escherichia coli - Part 1: Colony-count technique at 44 °C using membranes and 5-bromo-4-chloro-3- indolyl beta-D-glucuronide

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Organization: ISO
Publication Date: 1 April 2018
Status: active
Page Count: 18
ICS Code (Food microbiology): 07.100.30
scope:

This document specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli by colony-count technique after resuscitation using membranes and incubation at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme β-glucuronidase[9][10][13][14][17][ 18][19][20]. It is applicable to

- products intended for human consumption,

- products intended for feeding animals,

- environmental samples in the area of food production and food handling, and

- samples from the primary production stage such as animal faeces, dust, and swabs.

WARNING - Some strains of Escherichia coli may grow poorly or not at all in media incubated at 44 °C. This includes strains of E. coli O157:H7 and O157:H-. Additionally, some strains of Escherichia coli, notably those belonging to serotype O157:H7, are mostly β-glucuronidase negative[11]. Consequently, some strains of E. coli, including pathogenic ones, will not be detected by this method. β-glucuronidase activity may also be exhibited at 44 °C by certain other members of the Enterobacteriaceae, notably Shigella[15] and Salmonella[16] .

Document History

ISO 16649-1
April 1, 2018
Microbiology of the food chain - Horizontal method for the enumeration of beta-glucuronidasepositive Escherichia coli - Part 1: Colony-count technique at 44 °C using membranes and 5-bromo-4-chloro-3- indolyl beta-D-glucuronide
This document specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli by colony-count technique after resuscitation using membranes and incubation at 44 °C on a...
April 15, 2001
Microbiology of Food and Animal Feeding Stuffs - Horizontal Method for the Enumeration of B-Glucuronidase-Positive Escherichia Coli - Part 1: Colony-Count Technique at 44 Degrees C Using Membranes and 5-Bromo-4-Chloro-3-Indolyl B-D-Glucuronide
This part of ISO 16649 specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli in products intended for human consumption or for the feeding of animals. It uses...

References

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