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CEN - EN ISO 7932

Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of presumptive Bacillus cereus - Colony-count technique at 30 °C

active, Most Current
Organization: CEN
Publication Date: 1 December 2004
Status: active
Page Count: 46
ICS Code (Food microbiology): 07.100.30
scope:

This International Standard specifies a horizontal method for the enumeration of viable presumptive Bacillus cereus by means of the colony-count technique at 30 °C. It is applicable to

- products intended for human consumption and the feeding of animals, and

- environmental samples in the area of food production and food handling.

NOTE 1 In order to have a practicable test method, the confirmatory stage has been restricted to the typical aspect on MYP agar and the haemolysis test. Thus the term "presumptive" has been introduced in order to acknowledge the fact that the confirmatory stage does not enable the distinction of B. cereus from other closely related but less commonly encountered Bacillus species, such as B. anthracis, B. thuringiensis, B. weihenstephanensis, B. mycoides. An additional motility test may help to differentiate B. cereus from B. anthracis in cases where the presence of the latter is suspected.

NOTE 2 The diversity within the Bacillus cereus group is large with 7 phylogenetic groups[21][22] and a growing number of species.

[21] GUINEBRETIERE, M.H., THOMPSON F.L., SOROKIN A., NORMAND P., DAWYNDT P., EHLING-SCHULZ M., SVENSSON B., SANCHIS V., NGUYEN-THE C., HEYNDRICKX M., DE VOS P. Ecological diversification in the Bacillus cereus Group. Environmental Microbiology. 2008, 10(4), pp. 851-865

[22] GUINEBRETIERE M.H., VELGE P., COUVERT O., CARLIN F., DEBUYSER M.L., NGUYEN-THE C. Ability of Bacillus cereus Group Strains To Cause Food Poisoning Varies According to Phylogenetic Affiliation (Groups I to VII) Rather than Species Affiliation. Journal of Clinical Microbiology. 2010, 48(9), pp. 3388-3391

Document History

EN ISO 7932
December 1, 2004
Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of presumptive Bacillus cereus - Colony-count technique at 30 °C
This International Standard specifies a horizontal method for the enumeration of viable presumptive Bacillus cereus by means of the colony-count technique at 30 °C. It is applicable to — products...
December 1, 2004
Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of presumptive Bacillus cereus - Colony-count technique at 30 degrees C
A description is not available for this item.
December 1, 1997
Microbiology - General Guidance for the Enumeration of Bacillus Cereus - Colony-Count Technique at 30 Degrees C
A description is not available for this item.

References

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