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DIN EN ISO 15216-1

Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1: Method for quantification (ISO 15216-1:2017)

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Organization: DIN
Publication Date: 1 July 2017
Status: active
Page Count: 58
ICS Code (Food microbiology): 07.100.30
scope:

This document specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.

This method is not validated for detection of the target viruses in other foodstuffs (including multicomponent foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.

Document History

DIN EN ISO 15216-1
October 1, 2021
Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1: Method for quantification (ISO 15216-1:2017 + Amd 1:2021) (includes Amendment A1:2021)
A description is not available for this item.
DIN EN ISO 15216-1/A1
September 1, 2020
Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1: Method for quantification - AMENDMENT 1 (ISO 15216-1:2017/DAM 1:2020); German and English version EN ISO 15216-1:2017/prA1:2020
A description is not available for this item.
DIN EN ISO 15216-1
July 1, 2017
Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1: Method for quantification (ISO 15216-1:2017)
This document specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables,...
DIN EN ISO 15216-1
September 1, 2015
Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Part 1: Method for quantification (ISO/DIS 15216-1:2015); German and English version prEN ISO 15216-1:2015
A description is not available for this item.

References

This document references:
ISO 17468 - Microbiology of the food chain - Technical requirements and guidance on establishment or revision of a standardized reference method
Published by ISO on June 15, 2016
This International Standard gives technical requirements and guidance on the establishment or revision of standardized reference methods for the analysis (detection or quantification) of...
This document references:
ISO 20838 - Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
Published by ISO on April 15, 2006
A description is not available for this item.
This document references:
ISO 22119 - Microbiology of food and animal feeding stuffs — Real-time polymerase chain reaction (PCR) for the detection of foodborne pathogens — General requirements and definitions
Published by ISO on July 15, 2011
This International Standard defines terms for the detection of food-borne pathogens in foodstuffs, and isolates obtained from them, using the polymerase chain reaction (PCR). This International...
This document references:
ISO 22174 - Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
Published by ISO on February 15, 2005
A description is not available for this item.
This document references:
ISO 5725-2 - Accuracy (trueness and precision) of measurement methods and results — Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
Published by ISO on December 1, 2019
This document — amplifies the general principles for designing experiments for the numerical estimation of the precision of measurement methods by means of a collaborative interlaboratory experiment;...
This document references:
ISO 7218 - Microbiology of food and animal feeding stuffs - General requirements and guidance for microbiological examinations
Published by ISO on August 15, 2007
This International Standard gives general requirements and guidance/options intended for three main uses: — implementation of ISO/TC 34/SC 9 or ISO/TC 34/SC 5 standards for detection or enumeration...
This document is referenced by:
DIN EN ISO 18593 - Microbiology of the food chain - Horizontal methods for surface sampling (ISO 18593:2018)
Published by DIN on October 1, 2018
This document specifies horizontal methods for sampling techniques using contact plates, stick swabs, sponges and cloths on surfaces in the food chain environment in order to detect and enumerate...
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