scope:

This document describes a method for testing the susceptibility of filamentous fungi (moulds) that cause invasive (Aspergillus spp., Fusarium spp., Rhizopus oryzae [R. arrhizus], Pseudallescheria boydii [Scedosporium apiospermum], Sporothrix schenckii, and other pathogenic moulds) and cutaneous (the dermatophytes Trichophyton, Microsporum, and Epidermophyton spp.) fungal infections to antifungal agents.^2-5,10 Addressed in this document are testing conditions including inoculum preparation and inoculum size, incubation time and temperature, medium formulation, and criteria end-point determination.^7-9 Quality control (QC) reference ranges are also provided.^6,11

This standard focuses on the fully defined synthetic medium RPMI-1640 for testing of moulds because of the suitability of this test medium for antifungal susceptibility testing of yeasts.^2,3,11,12

Refer to CLSI document M27¹ for drug stock solution preparation and dilution procedures.