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DIN EN ISO 16649-3

Microbiology of the food chain - Horizontal method for the enumeration of ss-glucuronidase positive Escherichia coli - Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-ss-D-glucuronide (ISO 16649-3:2015); German version EN ISO 16649-3:2015

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Organization: DIN
Publication Date: 1 September 2015
Status: inactive
Page Count: 19
ICS Code (Food microbiology): 07.100.30
scope:

Dieser Teil von ISO 16649 legt ein horizontales Verfahren für den Nachweis und die Zählung von β-Glucuronidase-positiven Escherichia coli durch Kultivierung im flüssigen Nährmedium und Berechnung der wahrscheinlichsten Keimzahl (en: most probable number, MPN) nach der Bebrütung bei (37 ± 1) °C und anschließend bei (44 ± 1) °C fest. Dieser Teil von ISO 16649 ist anwendbar auf:

- Erzeugnisse, die für den menschlichen Verzehr und zur Fütterung von Tieren bestimmt sind;

- Umgebungsproben im Bereich der Herstellung und Handhabung von Lebensmitteln.

Das Verfahren ist geeignet für die Zählung von Zellen von E. coli, die möglicherweise Stress ausgesetzt waren, bedingt durch Austrocknung, Gefrieren und dem Ausgesetztsein einer salzhaltigen (z. B. marinen) Umgebung oder Beschädigung durch Desinfektionsmittel, wie z. B. chlorhaltige Produkte.

Eine Anwendungsbeschränkung dieses Teiles der ISO 16649 wird durch die Anfälligkeit des Verfahrens für ein hohes Maß an Variabilität bedingt. Das Verfahren und die Auswertung der Ergebnisse sind unter Berücksichtigung der in Abschnitt 11 angeführten Informationen durchzuführen.

Dieses Verfahren wurde noch nicht vollständig für alle Matrizes bewertet, z. B. für Milch und Milchprodukte. Für Milch und Milchprodukte ist ISO 7251 vorgesehen.

Document History

DIN EN ISO 16649-3
January 1, 2018
Microbiology of the food chain - Horizontal method for the enumeration of ss-glucuronidase positive Escherichia coli - Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-ss-D-glucuronide (ISO 16649-3:2015, Corrected version 2016-12-15); German version EN ISO 16649-3:2015
Dieser Teil von ISO 16649 legt ein horizontales Verfahren für den Nachweis und die Zählung von β-Glucuronidase-positiven Escherichia coli durch Kultivierung im flüssigen Nährmedium und Berechnung der...
DIN EN ISO 16649-3
September 1, 2015
Microbiology of the food chain - Horizontal method for the enumeration of ss-glucuronidase positive Escherichia coli - Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-ss-D-glucuronide (ISO 16649-3:2015); German version EN ISO 16649-3:2015
Dieser Teil von ISO 16649 legt ein horizontales Verfahren für den Nachweis und die Zählung von β-Glucuronidase-positiven Escherichia coli durch Kultivierung im flüssigen Nährmedium und Berechnung der...
DIN EN ISO 16649-3
May 1, 2013
Microbiology of food and animal feeding stuffs - Enumeration of β-glucuronidase-positive Escherichia coli - Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (ISO/DIS 16649-3:2013); German version prEN ISO 16649-3:2013
A description is not available for this item.

References

This document references:
ISO 11133 - Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media
Published by ISO on May 15, 2014
This International Standard defines terms related to quality assurance of culture media and specifies the requirements for the preparation of culture media intended for the microbiological analysis...
This document references:
ISO 6887-1 - Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions
Published by ISO on March 1, 2017
This document defines general rules for the aerobic preparation of the initial suspension and of dilutions for microbiological examinations of products intended for human or animal consumption. This...
This document references:
ISO 6887-2 - Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 2: Specific rules for the preparation of meat and meat products
Published by ISO on March 1, 2017
This document specifies rules for the preparation of meat and meat product samples and their suspension for microbiological examination when the samples require different preparation from the methods...
This document references:
ISO 6887-3 - Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products AMENDMENT 1: Sample preparation for raw marine gastropods
Published by ISO on February 1, 2020
A description is not available for this item.
This document references:
ISO 6887-4 - Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 4: Specific rules for the preparation of miscellaneous products
Published by ISO on March 1, 2017
This document specifies rules for the preparation of samples and dilutions for the microbiological examination of specific food products not covered in other parts of ISO 6887, which deal with more...
This document references:
ISO 6887-5 - Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 5: Specific rules for the preparation of milk and milk products
Published by ISO on April 1, 2020
This document specifies rules for the preparation of samples of milk and milk products and their suspensions for microbiological examination when the samples require a different preparation from the...
This document references:
ISO 6887-6 - Microbiology of food and animal feed - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 6: Specific rules for the preparation of samples taken at the primary production stage
Published by ISO on March 1, 2013
This part of ISO 6887 specifies rules for the preparation of samples taken at all stages from the farm to the slaughterhouse and their suspension for microbiological examination when the samples...
This document references:
ISO 7218 - Microbiology of food and animal feeding stuffs - General requirements and guidance for microbiological examinations
Published by ISO on August 15, 2007
This International Standard gives general requirements and guidance/options intended for three main uses: — implementation of ISO/TC 34/SC 9 or ISO/TC 34/SC 5 standards for detection or enumeration...
This document references:
7251 AMD 1 - Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of presumptive Escherichia coli — Most probable number technique AMENDMENT 1: Inclusion of performance testing of culture media and reagents
Published by ISO on October 1, 2023
A description is not available for this item.
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